Rao · Indian heart journal 2021 · cross-sectional study · n=80

Molecular analysis shows the presence of periodontal bacterial DNA in atherosclerotic plaques from patients with coronary artery disease.

Cited 20 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional observational study analyzing tissue samples from a single patient cohort without a non-atherosclerotic control group.

PubMed 33865522 · doi:10.1016/j.ihj.2021.01.011 · record verified 2026-08-29

What was done

Nested PCR was used to test for DNA from three periodontal pathogens (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) in subgingival and atheromatous plaque samples collected from 80 patients scheduled for coronary artery bypass graft (CABG) or angioplasty.

What was found

Pathogen DNA was detected in atherosclerotic plaque samples at the following frequencies: Tannerella forsythia in 12.5%, Porphyromonas gingivalis in 10%, and Treponema denticola in 1.3%. Patients whose atherosclerotic plaques tested positive for one or more pathogens all had chronic periodontitis.

Why it matters

This study provides molecular evidence that specific oral periodontal pathogen DNA can be found directly within coronary atheromatous plaques, supporting the plausibility of oral-vascular bacterial links.

Limits

The sample size is relatively small (n = 80), and the abstract does not report a non-atherosclerotic control group. Detection of bacterial DNA via PCR does not establish viability or active infection, cannot prove causation in plaque development, and subgingival pathogen percentages are not reported in the abstract.

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