Evans · Gut 1988 · observational physiological study · n=66

Measurement of gastrointestinal pH profiles in normal ambulant human subjects.

Cited 1245 times in the scientific literature.

Level 4 - case-series / case-control

Prospective observational physiological study / case series in healthy volunteers without a comparator group

PubMed 3410329 · doi:10.1136/gut.29.8.1035 · record verified 2026-08-29

What was done

Gastrointestinal pH was measured in 66 normal ambulant subjects for up to 48 hours using an ingested pH-sensitive radiotelemetry capsule and a portable solid-state recording receiver. Capsule location within the gastrointestinal tract was tracked using an external directional surface detector.

What was found

Gastric pH ranged from 1.0 to 2.5 in all subjects. Mean (SD) pH values across the gastrointestinal tract were: - Proximal small intestine (first hour): 6.6 (0.5) - Terminal ileum: 7.5 (0.4) (p < 0.001) - Caecum: 6.4 (0.4), showing a sharp drop upon entry in all subjects (p < 0.001) - Left colon: 7.0 (0.7), following a progressive rise from the right colon (p < 0.001)

Why it matters

This study provides normative reference values for regional gastrointestinal pH gradients in freely moving healthy humans, directly informing the design and evaluation of pH-dependent oral drug delivery formulations.

Limits

The study is purely descriptive without comparison to patients with gastrointestinal pathology. The abstract does not report participant demographics such as age and sex, nor does it describe dietary controls during the 48-hour ambulatory recording period.

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