De Miguel · Nature 2021 · preclinical animal study and human prospective cohort · n=?

Exercise plasma boosts memory and dampens brain inflammation via clusterin.

Cited 432 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal transfer experiments combined with preliminary human cohort biomarker data

PubMed 34880498 · doi:10.1038/s41586-021-04183-x · record verified 2026-08-26

What was done

Researchers collected plasma from voluntarily running mice ('runner plasma') and infused it into sedentary mice, assessing baseline and experimentally induced brain inflammation. Plasma proteomic profiling was conducted to identify circulating anti-inflammatory factors. They then intravenously administered clusterin (CLU), a complement cascade inhibitor identified in the screen, to mouse models of acute brain inflammation and Alzheimer's disease to measure endothelial binding and neuroinflammatory gene expression. Additionally, they measured plasma CLU levels in patients with cognitive impairment who completed a 6-month structured exercise intervention.

What was found

Infusion of runner plasma reduced baseline neuroinflammatory gene expression and experimentally induced brain inflammation in sedentary mice. Plasma proteomics showed an increase in complement cascade inhibitors, specifically CLU. Intravenously administered CLU bound to brain endothelial cells and decreased neuroinflammatory gene expression in both acute inflammation and Alzheimer's disease mouse models. Patients with cognitive impairment who completed 6 months of structured exercise showed higher plasma CLU levels. The abstract reports no numerical values or effect sizes.

Why it matters

This study identifies clusterin as a transferable exercise-induced blood factor that targets cerebrovascular endothelium to reduce neuroinflammation, providing a potential mechanism for exercise-induced cognitive protection.

Limits

The abstract provides no exact sample sizes for the mouse experiments or the human cohort. The core mechanistic findings rely on mouse models, which may not directly translate to human clinical outcomes. Long-term functional cognitive endpoints and clinical trial data in humans are not reported in the abstract.

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