Wang · Food chemistry 2022 · in vitro digestion model · n=?

Effect of gastrointestinal alterations mimicking elderly conditions on in vitro digestion of meat and soy proteins.

Cited 47 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

In vitro laboratory study with no human subjects

PubMed 35183956 · doi:10.1016/j.foodchem.2022.132465 · record verified 2026-08-29

What was done

Researchers evaluated the digestion of meat proteins (chicken, beef, and pork) and soy proteins using an in vitro simulated gastrointestinal digestion model. The model compared standard adult digestive conditions (control) with conditions simulating elderly individuals with achlorhydria (decreased gastric fluid secretion). Outomes evaluated included degree of hydrolysis (DH), SDS-PAGE electrophoresis profiles, peptide concentrations, and proteomic profiles across digestive phases.

What was found

The abstract reports no numerical values, effect sizes, or p-values. Digestion under simulated elderly achlorhydria conditions decreased the degree of protein hydrolysis, with the reduction being more pronounced for soy proteins. Proteomics and SDS-PAGE showed that meat myofibrillar/sarcoplasmic proteins and soy glycinin/beta-conglycinin were the most affected. The digestibility difference between elderly and adult conditions narrowed by the intestinal phase for meat proteins, whereas the impaired digestibility persisted into the intestinal phase for soy proteins.

Why it matters

The findings suggest that age-related decline in gastric acid secretion may impair plant (soy) protein digestion more substantially than animal meat protein digestion, which may be relevant when formulating dietary guidelines for older populations.

Limits

This is strictly an in vitro benchtop model that cannot fully capture in vivo gastrointestinal physiology, transit times, or mucosal absorption. No living human or animal subjects were evaluated. The abstract provides only qualitative directional descriptions without exact numerical metrics, variance, or statistical significance.

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