Chondronasiou · Aging cell 2022 · Controlled animal laboratory study · n=?

Multi-omic rejuvenation of naturally aged tissues by a single cycle of transient reprogramming.

Cited 138 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal research without human data.

PubMed 35235716 · doi:10.1111/acel.13578 · record verified 2026-08-31

What was done

Naturally aged mice were subjected to a single period of transient OSKM (OCT4, SOX2, KLF4, and MYC) pluripotency factor expression. The researchers measured multi-omic responses across the DNA methylome, transcriptome, and metabolome in multiple organs and serum.

What was found

The abstract reports qualitative multi-omic shifts without providing numerical values, sample sizes, or statistical effect estimates. A single cycle of transient OSKM expression reversed age-associated DNA methylation patterns in the pancreas, liver, spleen, and blood. It also reversed age-related transcriptional changes in relevant biological pathways and restored certain serum metabolites and biomarkers toward youthful levels.

Why it matters

This study shows that a single short burst of partial cellular reprogramming is sufficient to induce multi-tissue epigenetic, transcriptomic, and metabolic rejuvenation in naturally aged mammals, expanding prior findings beyond progeroid or injury models.

Limits

The findings are restricted to a mouse model, and translatability to humans remains unproven. The abstract does not disclose sample sizes, treatment duration, durability of the rejuvenated state, functional physiological outcomes, or numerical effect sizes.

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