Whittaker · Nutrition and health 2022 · systematic review and meta-analysis · n=27 studies (309 participants)

Low-carbohydrate diets and men's cortisol and testosterone: Systematic review and meta-analysis.

Cited 34 times in the scientific literature.

Level 1 - systematic review of randomized trials

Systematic review and meta-analysis of dietary intervention trials

PubMed 35254136 · doi:10.1177/02601060221083079 · record verified 2026-08-31

What was done

A systematic review and random-effects meta-analysis (PROSPERO CRD42021255957) searched eight databases through May 2021 to examine the effects of low-carbohydrate (≤35% carbohydrate) versus high-carbohydrate diets on testosterone and cortisol in healthy adult men. Cochrane's risk of bias tool was used for quality assessment. Subgroup analyses were conducted for diet duration (<3 weeks vs. ≥3 weeks), protein intake (<35% vs. ≥35%), and exercise duration.

What was found

Twenty-seven studies with 309 total participants were analyzed: - Short-term (<3 weeks) low-carbohydrate diets increased resting cortisol (SMD 0.41, 95% CI [0.16, 0.66], p < 0.01), whereas long-term (≥3 weeks) diets had no consistent effect on resting cortisol. - Following long-duration exercise (≥20 min), low-carbohydrate diets elevated post-exercise cortisol at 0 h (SMD 0.78 [0.47, 1.1], p < 0.01), 1 h (SMD 0.81 [0.31, 1.31], p < 0.01), and 2 h (SMD 0.82 [0.33, 1.3], p < 0.01). - Moderate-protein (<35%) low-carbohydrate diets had no consistent effect on resting total testosterone. - High-protein (≥35%) low-carbohydrate diets substantially decreased resting total testosterone (SMD -1.08 [-1.67, -0.48], p < 0.01; ~5.23 nmol/L) and post-exercise total testosterone (SMD -1.01 [-2.00, -0.01], p = 0.05).

Why it matters

The findings demonstrate that resting cortisol elevations from low-carbohydrate intake are transient while exercise-induced cortisol increases persist, and that reductions in testosterone are driven by high protein intake rather than carbohydrate restriction alone.

Limits

The total sample size across 27 studies was small (309 participants, averaging ~11 participants per study). The review was restricted to healthy adult males, and the abstract does not report data on free testosterone, blinding, adherence, or clinical health endpoints.

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