Acosta-Rodríguez · Science (New York, N.Y.) 2022 · controlled animal experiment · n=?

Circadian alignment of early onset caloric restriction promotes longevity in male C57BL/6J mice.

Cited 377 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal research (controlled rodent study).

PubMed 35511946 · doi:10.1126/science.abk0297 · record verified 2026-08-30

What was done

Male C57BL/6J mice were placed on a 30% caloric restriction (CR) regimen to evaluate how caloric intake, fasting intervals, and circadian alignment of feeding interact to influence lifespan. Hepatic gene expression associated with inflammation and metabolic pathways was also analyzed during aging in ad libitum-fed versus nighttime CR mice.

What was found

A 30% caloric restriction alone increased lifespan by 10%. When combined with a daily fasting interval and circadian alignment of feeding (nighttime feeding), lifespan was extended by 35% in male C57BL/6J mice, an effect independent of body weight. Nighttime CR also ameliorated age-related increases in liver inflammatory gene expression and decreases in metabolic pathway gene expression.

Why it matters

This study demonstrates that the longevity benefits of caloric restriction in mice depend heavily on the circadian timing of food intake and fasting intervals rather than calorie reduction alone.

Limits

The study was conducted exclusively in male C57BL/6J mice, and specific sample sizes and variance metrics are omitted from the abstract. Findings in nocturnal rodents cannot be directly translated to human circadian biology and longevity without clinical trials.

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