Rosas-Plaza · Frontiers in microbiology 2022 · pooled cross-sectional observational study · n=568

Human Gut Microbiome Across Different Lifestyles: From Hunter-Gatherers to Urban Populations.

Cited 69 times in the scientific literature.

Level 4 - case-series / case-control

Secondary pooled analysis of cross-sectional observational datasets

PubMed 35558108 · doi:10.3389/fmicb.2022.843170 · record verified 2026-08-29

What was done

The authors compiled and compared 16S rRNA gut microbiome sequencing data from 14 published studies comprising 568 individuals globally. Populations were classified along a lifestyle gradient into hunter-gatherer, agricultural, agropastoral, pastoral, and urban categories to evaluate differences in microbial alpha diversity, beta diversity, and taxonomic composition.

What was found

No numerical values, test statistics, or p-values are reported in the abstract. Industrialized populations showed lower diversity than non-industrialized groups. Traditional populations exhibited distinct alpha and beta diversity across lifestyle subcategories. Beta diversity analyses indicated that pastoral and agropastoral gut microbiomes were more similar to urban microbiomes than hunter-gatherer microbiomes were, representing a compositional transition toward industrialized states. Bacterial families including Bacteroidaceae, Lachnospiraceae, and Rikenellaceae were reported as differentially abundant in urban populations.

Why it matters

Subdividing traditional lifestyles reveals an ecological gradient in the gut microbiome that is masked when grouping all non-industrialized populations together. This helps delineate how specific subsistence transitions, such as pastoralism and agriculture, correlate with microbiome remodeling prior to full industrialization.

Limits

The abstract provides no quantitative metrics or effect sizes. Aggregating 16S data across 14 independent studies introduces potential technical batch effects and sequencing protocol variability. Cross-sectional observational data cannot establish causality or isolate specific dietary factors from other environmental and cultural exposures.

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