Li · Nature 2022 · Preclinical animal and bench experimental study with human and equine observational cohorts · n=?

An exercise-inducible metabolite that suppresses feeding and obesity.

Cited 329 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal and bench research with descriptive human biomarker validation

PubMed 35705806 · doi:10.1038/s41586-022-04828-5 · record verified 2026-08-26

What was done

Researchers investigated the biosynthetic pathway and physiological function of N-lactoyl-phenylalanine (Lac-Phe) during exercise. In diet-induced obese mice, they measured the effects of pharmacological Lac-Phe administration on food intake, movement, energy expenditure, adiposity, body weight, and glucose homeostasis, as well as the effects of genetic ablation of Lac-Phe biosynthesis during exercise training. They also evaluated activity-induced circulating Lac-Phe levels in humans and racehorses.

What was found

No quantitative values or effect sizes are reported in the abstract. Directionally, exercise stimulated Lac-Phe production synthesized by CNDP2+ cells. In diet-induced obese mice, pharmacological Lac-Phe reduced food intake without altering movement or energy expenditure, and chronic administration decreased adiposity, reduced body weight, and improved glucose homeostasis. Genetic ablation of Lac-Phe biosynthesis increased food intake and obesity following exercise. Activity-inducible increases in circulating Lac-Phe were confirmed in humans and racehorses.

Why it matters

This work identifies Lac-Phe as a conserved exercise-inducible signaling metabolite that regulates food intake and energy balance, revealing a molecular mechanism for exercise-mediated appetite suppression.

Limits

The abstract provides no sample sizes, participant demographics, dosage details, or numerical effect sizes. Direct causal effects of Lac-Phe on appetite suppression and weight loss were tested only in mice; human and equine data were limited to observational biomarker measurements after exertion.

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