The PICLS high-throughput screening method for agents extending cellular longevity identifies 2,5-anhydro-D-mannitol as novel anti-aging compound.
Level 5 - mechanism / opinion, no new human data
In vitro yeast model development and compound screening (CEBM Level 5).
PubMed 35705837 · doi:10.1007/s11357-022-00598-0
What was done
The authors developed a 96-well microplate method called PICLS to measure chronological lifespan in the yeast Saccharomyces cerevisiae. The protocol combines propidium iodide fluorescence to measure cell survival and optical density at 600 nm to measure total cell count from the same plate. The assay was validated with rapamycin and caloric restriction, then used to screen chemical compounds for effects on stationary-phase cellular survival.
What was found
The method enables high-throughput quantitative screening of yeast chronological lifespan. Application of the assay identified 2,5-anhydro-D-mannitol (2,5-AM) as having anti-aging and geroprotective properties in yeast. The abstract reports no numerical values, effect sizes, statistical parameters, or survival metrics.
Why it matters
The study presents a high-throughput platform for screening cellular longevity compounds and highlights 2,5-anhydro-D-mannitol as a novel candidate for aging research.
Limits
The study is restricted to an in vitro single-celled yeast model, limiting direct applicability to complex mammalian aging. The abstract provides no quantitative data on the magnitude of lifespan extension, dose response, or toxicity, and includes no validation in multicellular organisms or human models.
Cited by
- supports Yeast cells used in aging research live for about 10 days before dying.