Zhang · Immunity & ageing : I & A 2022 · Preclinical animal model and in vitro cell culture study · n=?

Aged microglia promote peripheral T cell infiltration by reprogramming the microenvironment of neurogenic niches.

Cited 64 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal and in vitro mechanistic study

PubMed 35879802 · doi:10.1186/s12979-022-00289-6 · record verified 2026-08-30

What was done

Researchers investigated the mechanisms of age-related peripheral T cell infiltration into the central nervous system. Using the subventricular zone of aged mice, cell-cell interaction analyses were conducted to evaluate microglial interactions with CD8+ T cells and brain venous endothelial cells. In addition, cultured human microglia were exposed in vitro to cerebrospinal fluid from elderly individuals.

What was found

The abstract reports qualitative mechanistic findings without exact numerical values: - Subventricular zones of aged mice exhibited chronic inflammation and peripheral T cell infiltration. - Aged microglia secreted CCL3, recruiting peripheral CD8+ memory T cells. - Pro-inflammatory aged microglia secreted TNF-α, which upregulated VCAM1 and ICAM1 expression on brain venous endothelial cells and facilitated T cell transendothelial migration. - In vitro human microglia treated with elderly cerebrospinal fluid adopted a chemotactic phenotype.

Why it matters

The study outlines a specific microglial pathway that reprograms brain vascular and neurogenic microenvironments to facilitate adaptive immune cell entry during aging.

Limits

No quantitative measurements, sample sizes, or statistical metrics are reported in the abstract. Findings are predominantly from rodent models and in vitro cultures, requiring validation in human brain tissue.

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