Anti-Obesogenic Effects of Sulforaphane-Rich Broccoli ( Brassica oleracea var. italica ) Sprouts and Myrosinase-Rich Mustard ( Sinapis alba L.) Seeds In Vitro and In Vivo.
Level 5 - mechanism / opinion, no new human data
Preclinical laboratory study using cell cultures and animal models (Level 5 by design analogy).
PubMed 36145190 · doi:10.3390/nu14183814
What was done
Researchers evaluated the anti-obesogenic effects of broccoli sprout powder (BSP), mustard seed powder (MSP), and a combined sulforaphane-rich mixture (MBP) in bisphenol A (BPA)-treated 3T3-L1 preadipocytes and BPA-induced obese C57BL/6J mice. They examined cell viability, intracellular lipid accumulation, body weight changes, lipid profiles, and markers of adipogenesis and lipolysis via protein expression analyses.
What was found
None of the powders demonstrated cytotoxicity in vitro. In 3T3-L1 cells, MBP and BSP significantly suppressed BPA-induced lipid accumulation. In obese mice, BSP and MBP reduced body weight gain and improved dyslipidemia, with MBP demonstrating greater efficacy than BSP alone. Both treatments activated AMPK phosphorylation and ACC phosphorylation, upregulated lipolysis markers (UCP-1, CPT-1), and downregulated adipogenic transcription factors and enzymes (PPAR-γ, C/EBP-α, SREBP-1c, FAS, aP2) in vitro and in vivo. Specific numerical values and effect sizes were not reported in the abstract.
Why it matters
Co-administering myrosinase-rich mustard seed with glucoraphanin-rich broccoli sprouts may enhance sulforaphane bioavailability and activity, suggesting a dietary strategy to counteract environmental toxicant-induced metabolic dysfunction.
Limits
The abstract reports no numerical data, confidence intervals, or sample sizes (n of animals or cell culture replicates). The findings are restricted to in vitro and rodent models of BPA-induced obesity and cannot be directly generalized to human obesity or standard high-fat diet models without clinical trials.
Cited by
- supports Animal studies demonstrate that administration of sulforaphane protects against bisphenol A (BPA) toxicity.