Sadowska · Nutrients 2022 · controlled animal experiment · n=50

The Effect of Alternating High-Sucrose and Sucrose Free-Diets, and Intermittent One-Day Fasting on the Estrous Cycle and Sex Hormones in Female Rats.

Cited 4 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal research in female rats

PubMed 36297033 · doi:10.3390/nu14204350 · record verified 2026-08-30

What was done

Fifty female rats were allocated across five feeding groups to test the effects of sucrose intake levels and patterns alongside intermittent fasting. Interventions included diets with sucrose at 9% or 18% of dietary energy content, alternating high-sucrose and sucrose-free diets, and a high-sucrose diet paired with intermittent one-day fasting. Outcomes assessed were estrous cycle parameters and concentrations of estradiol, progesterone, and luteinizing hormone.

What was found

The abstract reports directional changes without exact numbers, variances, or p-values. Sucrose at 9% of dietary energy increased estradiol and decreased progesterone concentrations. A high-sucrose diet, even when alternating with a sucrose-free diet, decreased luteinizing hormone. Combining high-sucrose feeding with one-day fasting caused the greatest hormonal alterations, shortened the estrous phase, and disrupted the estrous cycle.

Why it matters

This study shows that both the amount and consumption pattern of dietary sucrose, including its combination with fasting, can alter female sex hormone profiles and ovarian cyclicity in rodents.

Limits

The study was conducted in female rats, limiting direct translation to human reproductive endocrinology. The abstract provides no quantitative hormone measurements, group-specific sample sizes, intervention durations, or statistical effect sizes.

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