Ibrahim · Journal of controlled release : official journal of the Controlled Release Society 2023 · preclinical animal experiment · n=?

Investigation of anti-PEG antibody response to PEG-containing cosmetic products in mice.

Cited 47 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal research

PubMed 36632951 · doi:10.1016/j.jconrel.2023.01.012 · record verified 2026-08-29

What was done

In a preclinical murine study, researchers evaluated whether low molecular weight PEG derivatives contained in two commercially available cosmetic products could penetrate the skin barrier and stimulate systemic anti-PEG immune responses. The investigators compared topical application on intact skin versus injured or compromised skin, tracked the emergence of anti-PEG IgM antibodies over time, and examined how topical cosmetic application affected antibody levels in mice with pre-induced circulating anti-PEG IgM.

What was found

Topically applied PEG derivatives penetrated the stratum corneum, entered systemic circulation, and stimulated anti-PEG IgM production. Skin penetration was enhanced in compromised skin. Anti-PEG IgM was detected by Day 14 in mice with normal skin and by Day 7 in mice with compromised skin. In mice with pre-existing circulating anti-PEG IgM, topically applied cosmetic PEG derivatives bound to the antibodies and lowered circulating blood levels. No specific sample sizes, concentrations, or numerical antibody titers were reported in the abstract.

Why it matters

Pre-existing anti-PEG antibodies are common in healthy humans who have never received intravenous PEGylated drugs. These findings demonstrate that topical exposure to everyday PEG-containing cosmetic products can trigger systemic anti-PEG antibody generation in mice, pointing to a potential environmental exposure route in humans.

Limits

This was an animal study; findings in mouse skin and murine immune models cannot be directly extrapolated to human skin permeability or human immune responses. The abstract does not disclose the sample size, specific product names, exact PEG concentrations, statistical significance values, or whether class-switched anti-PEG IgG antibodies developed.

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