Sundar · The Saudi dental journal 2023 · Systematic review of preclinical studies · n=23 studies

The role of Vitamin D as an adjunct for bone regeneration: A systematic review of literature.

Cited 27 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Systematic review limited entirely to preclinical in vitro and animal studies

PubMed 37091280 · doi:10.1016/j.sdentj.2023.02.002 · record verified 2026-08-28

What was done

Authors conducted a systematic review searching indexed literature from January 1990 to June 2022 to assess adjunctive vitamin D in bone defect regeneration, osseointegration, and in vitro osteogenic differentiation. From 384 identified titles, 23 preclinical studies were included (9 in vitro and 14 in vivo animal studies). No human clinical trials were included.

What was found

The abstract reports directional findings without numerical values, effect sizes, or confidence intervals. In vitro, adjunctive vitamin D with stem cells and osteoblasts enhanced osteogenic differentiation and upregulated alkaline phosphatase and bone matrix protein gene expression. In vivo animal models demonstrated accelerated and increased new bone formation and mineralization in defects, improved bone-implant contact, and enhanced osseointegration, even in animals with induced systemic illnesses. Systemic administration showed superior healing outcomes compared to local delivery.

Why it matters

This review aggregates preclinical biological evidence supporting vitamin D as a low-cost adjunct to enhance bone healing and osseointegration, pointing to specific parameters needing evaluation in future clinical trials.

Limits

The evidence base is entirely preclinical (in vitro cell cultures and animal models), limiting direct translation to clinical human efficacy. The abstract provides no quantitative data, effect estimates, or assessments of study quality or publication bias.

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