Rosenberg · Nature communications 2023 · Animal neurobehavioral and biochemical mapping study · n=?

Brain mitochondrial diversity and network organization predict anxiety-like behavior in male mice.

Cited 60 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal research measuring mouse brain tissue biochemistry and behavior

PubMed 37563104 · doi:10.1038/s41467-023-39941-0 · record verified 2026-08-26

What was done

Researchers measured mitochondrial respiratory chain enzyme activities and mitochondrial DNA (mtDNA) content across 571 tissue samples from 17 brain regions in male mice with diverse behavioral phenotypes. They applied multi-slice network analysis to map brain-wide mitochondrial organization and evaluated correlations with stress-related/anxiety-like behaviors, regional gene expression patterns, and structural connectivity.

What was found

Multi-slice network analysis identified three large-scale brain networks with shared mitochondrial signatures. A dominant network composed of cortico-striatal regions exhibited the strongest correlation with behavior, accounting for up to 50% of animal-to-animal behavioral differences. Specific enzyme activity rates, mtDNA content numbers, and correlation coefficients were not reported in the abstract.

Why it matters

This study provides evidence that regional mitochondrial energy transformation capacity is organized into discrete, functionally significant brain networks that strongly correlate with individual variation in anxiety-like behavior.

Limits

The study was conducted exclusively in male mice, so potential sex-specific patterns were not examined. The total number of individual mice is not reported in the abstract (only 571 samples across 17 brain areas). As an animal correlation study, it does not demonstrate causal manipulation of anxiety phenotypes, and findings may not directly translate to human neuropsychiatric conditions.

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