Danhof · Gut microbes 2023 · In vitro and ex vivo mechanistic laboratory study · n=?

Microbial stimulation of oxytocin release from the intestinal epithelium via secretin signaling.

Cited 50 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Bench research using human and mouse tissues, organoids, and single-cell sequencing datasets

PubMed 37698879 · doi:10.1080/19490976.2023.2256043 · record verified 2026-08-29

What was done

Researchers analyzed single-cell RNA-sequencing datasets and imaged human and mouse intestinal tissues to map intestinal oxytocin production. They utilized human intestinal organoids and human intestinal tissue models to test whether the probiotic bacterium Limosilactobacillus reuteri stimulates oxytocin secretion and to determine the signaling pathway involved.

What was found

The abstract reports no numerical data, effect sizes, or statistical metrics. Qualitatively, oxytocin was found to be produced directly by enterocytes in human and mouse intestinal epithelium and by human intestinal organoids. Exposure to L. reuteri stimulated oxytocin release from human intestinal tissue and organoids via a pathway dependent on secretin released from enteroendocrine cells.

Why it matters

This establishes the gut epithelium as an endogenous source of oxytocin and delineates a specific cellular mechanism by which commensal bacteria can trigger hormone release relevant to gut and systemic health.

Limits

The abstract contains no sample sizes, effect estimates, or variance statistics. Evidence is limited to cell culture, organoid models, and tissue specimens, and clinical in vivo effects in humans remain to be established.

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