Sang · Cell 2023 · controlled laboratory animal experiment · n=?

Prolonged sleep deprivation induces a cytokine-storm-like syndrome in mammals.

Cited 257 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal and bench mechanistic research in mice

PubMed 38016470 · doi:10.1016/j.cell.2023.10.025 · record verified 2026-08-26

What was done

Researchers evaluated a sleep deprivation paradigm called curling prevention by water in mice to persistently suppress both rapid eye movement sleep and non-rapid eye movement sleep. They assessed survival, inflammatory response, and the mechanistic role of brain prostaglandin D2, the ABCC4 transporter, and the PGD2/DP1 signaling pathway.

What was found

Mice remained awake 96% of the time. After 4 days of exposure, mice exhibited severe inflammation and approximately 80% died. Elevated brain prostaglandin D2 crossed the blood-brain barrier via the ATP-binding cassette subfamily C4 transporter, driving circulating neutrophil accumulation and a cytokine-storm-like syndrome. Experimental disruption of the PGD2/DP1 axis reduced this inflammation. Specific sample sizes and quantitative biomarker levels were not reported in the abstract.

Why it matters

The study outlines a direct molecular pathway linking profound sleep loss to fatal peripheral immune activation via central nervous system prostaglandin efflux.

Limits

Findings are limited to mice subjected to extreme acute sleep loss and cannot be directly translated to human sleep restriction. Total animal numbers, control group baseline values, and specific cytokine measurements were not provided in the abstract.

Cited by