Li · The Science of the total environment 2024 · cross-sectional study · n=40

Prevalence and implications of microplastic contaminants in general human seminal fluid: A Raman spectroscopic study.

Cited 97 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional observational study without an unexposed control group.

PubMed 38802004 · doi:10.1016/j.scitotenv.2024.173522 · record verified 2026-08-29

What was done

Semen samples were collected from 40 men without occupational exposures undergoing premarital health assessments in Jinan, China. Researchers used Raman microspectroscopy to detect, quantify, and identify polymer types of microplastics ranging in size from 0.72 to 7.02 μm. Computer-assisted sperm analysis evaluated motility, Diff-Quik staining assessed morphology, and statistical analyses tested associations between microplastic polymers, demographics, and semen parameters.

What was found

Microplastics were detected in 100% of the 40 semen samples, with an average of 2 particles per sample across eight polymer types. Polystyrene was the most common polymer (31%). Semen containing polystyrene exhibited higher progressive motility compared to semen containing polyvinyl chloride (43.52 ± 14.21% vs 19.04 ± 13.46%). Sperm morphological abnormalities were observed across samples but did not show statistically significant associations with specific polymer types.

Why it matters

This study demonstrates that microplastic exposure is detectable in the seminal fluid of general-population individuals without occupational risk. It suggests that specific plastics, notably polyvinyl chloride, may correlate with reduced sperm progressive motility.

Limits

The sample size was small (40 participants) from a single geographic region. The cross-sectional design cannot establish causality or determine the timing and route of exposure. Particle counts were low (averaging 2 particles per sample), increasing susceptibility to procedural contamination or measurement error. Confounding lifestyle and environmental variables were not fully reported in the abstract.

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