Kane · bioRxiv : the preprint server for biology 2024 · Controlled animal interventional study · n=?

Long-term NMN treatment increases lifespan and healthspan in mice in a sex dependent manner.

Cited 18 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal interventional study.

PubMed 38979132 · doi:10.1101/2024.06.21.599604 · record verified 2026-08-27

What was done

Researchers evaluated the effects of long-term administration of the NAD+ precursor nicotinamide mononucleotide (NMN) on male and female mice, assessing lifespan, frailty, physical activity, gene expression patterns, adipose accumulation, metabolic health, gut microbiota, and tumor burden/severity.

What was found

In both male and female mice, chronic NMN treatment increased physical activity, preserved more youthful gene expression, reduced frailty, and enriched levels of the gut bacterium *Anaerotruncus colihominis* without increasing tumor counts or severity. Effects on metabolism and longevity showed clear sex specificity: NMN slowed late-life adipose accumulation and improved metabolic health in male mice but not females, whereas female mice experienced an 8.5% increase in median lifespan that was not observed in males.

Why it matters

This study provides evidence that chronic NMN treatment can extend mammalian lifespan and delay frailty in a sex-dependent manner without accelerating tumorigenesis, highlighting the need to evaluate NAD+ boosters across both sexes.

Limits

The abstract does not state the sample sizes, mouse strain, dosage, or route/duration of NMN administration. The findings are restricted to a mouse model, have not been demonstrated in humans, and are reported in a preprint that has not undergone formal peer review.

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