McClure · Experimental physiology 2024 · Single-blind randomized crossover trial · n=16

Ketone monoester attenuates oxygen desaturation during weighted ruck exercise under acute hypoxic exposure but does not impact cognitive performance.

Cited 10 times in the scientific literature.

Level 2 - randomized trial

Randomized crossover trial in humans

PubMed 39190570 · doi:10.1113/EP091789 · record verified 2026-08-29

What was done

In a single-blind randomized crossover study, 16 males underwent cognitive assessments before and during acute normobaric hypoxia (simulated altitude of 4,572 m, 11.8% O2) combined with moderate exercise (two 20-minute weighted ruck bouts with ~22 kg at 3.2 km/h on a 10% incline). Participants consumed either an (R)-3-hydroxybutyl (R)-3-hydroxybutyrate ketone monoester (573 mg/kg) or a calorie- and taste-matched placebo (~50 g maltodextrin), both co-ingested with 40 g of dextrose prior to hypoxia exposure. Measured outcomes included blood R-βHB concentrations, oxygen saturation, and cognitive tests (Defense Automated Neurobehavioral Assessment code substitution, Stroop color and word test, and a shooting simulation).

What was found

Ketone monoester ingestion rapidly increased and sustained R-βHB concentrations above 3 mM (P < 0.001). Ketone monoester attenuated the decline in blood oxygen saturation during hypoxic exposure by 2.4% to 4.2% compared with placebo (P < 0.05). However, cognitive performance on the DANA code substitution task, Stroop task, and shooting simulation did not differ significantly between ketone monoester and placebo trials before or during hypoxic exposure.

Why it matters

Exogenous ketosis can modestly preserve peripheral oxygen saturation during acute severe hypoxic stress and loaded physical exertion, though this physiological benefit did not confer short-term cognitive or marksmanship advantages.

Limits

The study evaluated a small sample of 16 participants, limited strictly to males. It examined acute, short-duration normobaric hypoxia, leaving effects during chronic hypobaric altitude exposure untested. The study was single-blinded rather than double-blinded.

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