Identification of a new human senescent skin cell marker ribonucleoside-diphosphate reductase subunit M2 B.
Level 5 - mechanism / opinion, no new human data
Bench research using in vitro cell models and ex vivo human skin tissue.
PubMed 39261410 · doi:10.1007/s10522-024-10135-5
What was done
Researchers evaluated ribonucleoside-diphosphate reductase subunit M2 B (RRM2B) as a senescent cell marker in human dermal fibroblasts subjected to replicative senescence, ionizing radiation, or UVB exposure. They measured RRM2B gene and protein expression alongside senescence-associated β-galactosidase activity, bromodeoxyuridine (BrdU) proliferation assays, and senescence-associated secretory phenotype (SASP) gene expression. RRM2B-positive cells were analyzed using flow cytometry, and RRM2B localization was assessed in aging human skin samples.
What was found
The abstract reports no numerical values. RRM2B was upregulated at the gene and protein levels in replicative-, radiation-, and UVB-induced fibroblast models, coinciding with decreased BrdU incorporation, increased senescence-associated β-galactosidase activity, and elevated SASP factor gene expression. Flow cytometry showed higher recovery of RRM2B-positive cells in senescent cell populations. In aging human skin, RRM2B was more abundant in the dermis and epidermal basal layer.
Why it matters
Identifying specific biomarkers like RRM2B helps characterize senescent cell accumulation in human skin and may facilitate targeted senolytic therapies for skin aging.
Limits
The abstract provides no sample sizes, numerical data, or statistical values. The findings are limited to in vitro cell models and observational tissue localization without functional in vivo or clinical outcome validation.
Cited by
- supports Cellular senescence occurs in the basal layer of keratinocytes in human skin.