Wu · Frontiers in nutrition 2024 · cross-sectional observational study · n=20,337

Dose-response relationship of dietary Omega-3 fatty acids on slowing phenotypic age acceleration: a cross-sectional study.

Cited 13 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional observational study using survey data.

PubMed 39296507 · doi:10.3389/fnut.2024.1424156 · record verified 2026-08-29

What was done

Researchers analyzed NHANES data from 1999 to 2018 comprising 20,337 adult participants to examine the relationship between dietary omega-3 fatty acid intake and PhenoAgeAccel (the difference between phenotypic biological age, calculated from blood biochemical markers, and chronological age). They used weighted multivariable generalized linear regression, restricted cubic splines, and piecewise regression to evaluate linear and non-linear dose-response relationships, threshold effects, and subgroup interactions.

What was found

After adjusting for potential confounders, dietary omega-3 intake was negatively associated with PhenoAgeAccel (beta = -0.071; 95% CI: -0.119 to -0.024; p = 0.004), corresponding to an average decrease of 0.071 units of accelerated phenotypic aging per unit increase in intake. Piecewise regression identified an intake threshold of 1.103 grams/day, above which the reduction in PhenoAgeAccel plateaued. Subgroup analyses showed that gender, age, race, and hypertension status significantly modified this association.

Why it matters

This study provides population-level evidence that modest dietary omega-3 intake is linked to slower biological aging, pointing to a potential inflection point at roughly 1.1 grams per day.

Limits

The cross-sectional design cannot establish temporal order or causality. Dietary omega-3 intake was derived from self-reported recall, which is vulnerable to recall bias and misclassification. Residual confounding from unmeasured lifestyle factors remains possible.

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