Shen · Cell stem cell 2024 · Preclinical animal model study · n=?

Expansion of the neocortex and protection from neurodegeneration by in vivo transient reprogramming.

Cited 22 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical bench and animal study without human data

PubMed 39426381 · doi:10.1016/j.stem.2024.09.013 · record verified 2026-08-26

What was done

Researchers induced Yamanaka factors (YFs) in mouse brains using spatiotemporally controlled methods across two paradigms: during embryonic brain development and during adulthood in the dorsal hippocampus of a 5xFAD Alzheimer's disease mouse model. They assessed cell identity, neural progenitor expansion, cortical neuron and glial cell counts, behavioral outcomes (motor and social behavior), and Alzheimer's disease pathology including cognitive decline and molecular signatures.

What was found

The abstract reports no numerical values, effect sizes, or confidence intervals. Qualitatively, transient low-level embryonic YF induction was tolerated and resulted in progenitor expansion, an increased number of upper cortical neurons and glia, and enhanced adult motor and social behaviors. In adult 5xFAD mice, controlled hippocampal YF expression was tolerated by principal neurons and prevented the development of several Alzheimer's disease hallmarks, including cognitive decline and altered molecular signatures.

Why it matters

This study demonstrates that controlled, transient in vivo cellular reprogramming can safely expand specific neural cell populations during neurogenesis and counteract neurodegenerative features in a mouse model of Alzheimer's disease.

Limits

The findings are derived entirely from mouse models and cannot be directly generalized to human physiology. The abstract provides no sample sizes, exact quantitative measures, effect sizes, or statistical metrics, and potential long-term safety risks such as tumorigenesis or off-target epigenetic disruption were not detailed in the abstract.

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