Murugesan · Frontiers in nutrition 2024 · non-randomized sequential within-subject trial · n=25

Food order affects blood glucose and insulin levels in women with gestational diabetes.

Cited 4 times in the scientific literature.

Level 3 - non-randomized controlled study

Non-randomized sequential within-subject interventional trial without randomized order or parallel control

PubMed 39777075 · doi:10.3389/fnut.2024.1512231 · record verified 2026-08-26

What was done

Twenty-five women with gestational diabetes mellitus (GDM) participated in a 4-week, three-phase trial: baseline (week 0), phase 1 (regular diet, weeks 0–2), and phase 2 (food-order intervention, weeks 2–4). In phase 2, participants followed customized meal plans consuming vegetables first, protein second, and carbohydrates last, tracked via a mobile app. Blood glucose and serum insulin were measured at fasting, 1 hour, and 2 hours following a standardized clinic meal (339 kcal, 16.4 g protein, 56.1 g carbohydrates, 3.4 g fat) at the end of each phase.

What was found

Compared to the regular diet phase, the food-order intervention significantly decreased postprandial blood glucose by 5.87% at 60 minutes (p = 0.001) and by 6.06% at 120 minutes (p = 0.001). Serum insulin levels were significantly reduced by 8.13% at 60 minutes (p = 0.001) and by 11.10% at 120 minutes (p = 0.001).

Why it matters

Sequencing meal components to consume fiber and protein before carbohydrates is a simple, behavioral dietary strategy that acutely blunts postprandial glucose and insulin excursions in gestational diabetes.

Limits

The study had a very small sample size (n = 25) and a short intervention window (2 weeks per phase). The fixed sequential design lacked a randomized order or parallel control group, leaving findings vulnerable to period or order effects. Clinical maternal and neonatal outcomes were not assessed.

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