Le · The American journal of pathology 2025 · Preclinical animal experimental model · n=?

Shared Phenotypes of Immune Cells Recruited to the Cornea and the Surface of the Lens in Response to Formation of Corneal Erosions.

Cited 4 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal/in vivo wounding model without human subjects

PubMed 39889825 · doi:10.1016/j.ajpath.2025.01.006 · record verified 2026-08-26

What was done

The authors used an experimental corneal debridement wounding model to characterize the immune cell phenotypes recruited to the cornea following erosion formation. They evaluated whether immune cells concurrently migrate to the anterior surface of the lens and characterized cell subtypes using lineage markers (including Ly6G, Ly6C, myeloperoxidase) and cytokine expression (IL-10).

What was found

The abstract reports no quantitative values, sample sizes, or statistical metrics. Qualitatively, corneal erosions triggered an influx of M2 macrophages and Ly6G+ Ly6C+ myeloperoxidase+ neutrophil/polymorphonuclear-myeloid-derived suppressor cell (PMN-MDSC)-like cells into the corneal stroma, with few regulatory T cells. A parallel recruitment of predominantly neutrophil/PMN-MDSC-like cells occurred on the anterior, cornea-facing lens capsule. Both corneal and lens-associated neutrophil/PMN-MDSC-like populations expressed the anti-inflammatory cytokine IL-10.

Why it matters

The study identifies an interconnected anterior segment immune response where the lens capsule concurrently recruits IL-10-producing myeloid cells alongside corneal wound repair, suggesting a broader regional mechanism to manage ocular inflammation.

Limits

This is an animal debridement model; applicability to human recurrent corneal erosions remains unverified. The abstract does not report the host species, sample size (n), quantitative cell counts, time-course metrics, or functional blockade experiments to confirm whether lens-associated cells are necessary for wound resolution or infection defense.

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