Medeiros · Journal of reproductive immunology 2025 · Controlled laboratory animal study · n=?

Involvement of CXCL12/CXCR4 pair in migration of thymocytes from lactating mice.

Cited 1 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Controlled animal and in vitro laboratory study

PubMed 39904071 · doi:10.1016/j.jri.2025.104444 · record verified 2026-08-26

What was done

Nulliparous female C57BL/6 mice were compared to female mice on the 15th day of lactation. Post-euthanasia, thymi were harvested, weighed, and examined via histological analysis and prolactin (PRL) quantification. Serum PRL levels were also measured. Fresh thymocytes isolated from disrupted thymi were analyzed via flow cytometry for CXCR4 expression and assessed for in vitro migration capacity in response to CXCL12.

What was found

On lactation day 15, mice showed reduced thymic weight and cellularity alongside typical lactation-associated histological changes. Thymic supernatant contained higher concentrations of PRL than matching plasma serum. The thymic medulla demonstrated increased CXCL12 levels, and isolated thymocytes showed higher CXCR4 expression and increased migration toward CXCL12. Specific numerical values, effect sizes, and statistical metrics were not reported in the abstract.

Why it matters

This study identifies a potential role for the CXCL12/CXCR4 chemokine axis in driving thymocyte migration and reorganization during physiological postpartum thymic recovery.

Limits

The abstract reports purely preclinical mouse data without specifying sample sizes, exact numerical values, or confidence intervals. Human translatability is unestablished, and direct causal manipulation of the prolactin-CXCL12/CXCR4 axis was not described in the abstract.

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