Mortazavi · BioImpacts : BI 2025 · Narrative review · n=?

Topically applied GHK as an anti-wrinkle peptide: Advantages, problems and prospective.

Cited 6 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Narrative review of mechanistic, formulation, and preclinical data without primary clinical evidence.

PubMed 39963574 · doi:10.34172/bi.30071 · record verified 2026-08-26

What was done

This narrative review examined the biological properties, anti-wrinkle efficacy, skin permeability, formulation hurdles, and permeation enhancement strategies for topical glycyl-histidyl-lysine (GHK) and its derivatives, including GHK-Cu (copper complex) and Pal-GHK (palmitoylated GHK).

What was found

The abstract reports no numerical values. Cellular evidence supports GHK's role in tissue regeneration, collagen and glycosaminoglycan synthesis, angiogenesis, and nerve outgrowth. However, the authors note a surprising lack of published clinical trials evaluating GHK-Cu and Pal-GHK in humans. Furthermore, native GHK faces delivery limitations due to its hydrophilic and unstable nature, though permeability is enhanced through metal complexation, hydrophobic chemical modification, cell-penetrating peptides, and microneedle pretreatment.

Why it matters

This review highlights that commercial anti-wrinkle claims for GHK and its derivatives rely primarily on cellular mechanisms rather than robust human clinical trials, while emphasizing the formulation challenges required to achieve adequate skin penetration.

Limits

The paper is a narrative review offering no original empirical data or quantitative synthesis. The abstract does not specify search criteria, study counts, or effect sizes, and it explicitly identifies an absence of published clinical efficacy studies in human populations.

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