Shekhar · European journal of endocrinology 2025 · within-subject crossover trial · n=19

Thyroid axis adaptations to moderate short-term energy restriction in healthy, young women.

Cited 0 times in the scientific literature.

Level 2 - randomized trial

Controlled crossover clinical trial in healthy volunteers (NCT02858336)

PubMed 40265345 · doi:10.1093/ejendo/lvaf083 · record verified 2026-08-26

What was done

Nineteen healthy, non-obese women aged 23.36 ± 2.08 years without thyroid disease completed two 5-day diet protocols during the early follicular phases of successive menstrual cycles: a neutral energy availability diet (NEA, ±0%) and a deficient energy availability diet (DEA, -55%). Blood was sampled every 10 minutes between 8 AM and 4 PM and analyzed for TSH, GH, and cortisol every 30 minutes, total T3 (TT3), reverse T3 (rT3), and total T4 (TT4) hourly, and free T3, free T4, and TBG at baseline and study completion. Liquid chromatography-tandem mass spectrometry assessed all thyroid hormones except TSH and TBG.

What was found

DEA produced a small reduction in body weight (1.1 ± 0.21 kg, P < .001) and BMI (0.4 ± 0.08 kg/m2, P < .001) with unchanged fat mass. Compared to NEA, DEA led to significant declines in TT3 (ls mean ± SEM: 89.15 ± 2.89 vs 95.55 ± 2.89 ng/dL, P < .0001) and TSH (0.92 ± 0.07 vs 1.03 ± 0.07 µIU/mL, P < .0001). Conversely, DEA increased TT4 (6.26 ± 0.25 vs 6.06 ± 0.25 µg/dL, P = .005), fT4 (1.83 ± 0.07 vs 1.71 ± 0.07 ng/dL, P = .0052), and rT3 (12.04 ± 0.56 vs 9.02 ± 0.56 ng/dL, P < .0001). No significant changes were seen in TBG, GH, or cortisol.

Why it matters

These findings show that both central down-regulation of the thyroid axis and peripheral shifting toward inactive reverse T3 occur rapidly within five days of moderate energy restriction in healthy, lean young women.

Limits

The sample size is small (n = 19) and restricted to young, healthy, lean females, limiting generalizability to men, older individuals, or people with obesity. The abstract does not report whether diet order was randomized, omits free T3 quantitative values, and only evaluates short-term 5-day restriction.

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