Dikel · Veterinary medicine and science 2025 · in vitro comparative extraction and chemical characterization study · n=?

Ultrasound-Assisted Extraction (UAE) for the Utilization of Deep-Water Pink Shrimp (Parapenaeus longirostris) Processing Waste: Comprehensive Characterization of Carotenoid Astaxanthin Rich Extract.

Cited 2 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Bench and in vitro extraction study with no human data (graded by design analogy).

PubMed 40644491 · doi:10.1002/vms3.70498 · record verified 2026-08-26

What was done

Astaxanthin was extracted from deep-water pink shrimp (Parapenaeus longirostris) shell waste using ultrasound-assisted extraction (UAE) across six solvents of varying polarity (petroleum ether, hexane, isopropanol, ethanol, methanol, acetone) at two time points (2 and 4 h). Extracts were quantified via high-performance liquid chromatography (HPLC) and characterized by UV-Vis, Fourier transform infrared (FTIR), and Raman spectroscopy compared to a commercial astaxanthin standard.

What was found

Higher polarity solvents produced higher astaxanthin yields. The highest concentration occurred after 2 h of extraction with methanol (310 ± 2.00 µg/g), while the lowest was observed after 4 h with petroleum ether (104 ± 30.00 µg/g) (p < 0.05). UV-Vis spectra confirmed characteristic maximum absorbance between 480 and 520 nm, and FTIR/Raman spectra verified the functional groups and conjugated polyene chain vibrations matching astaxanthin.

Why it matters

This study demonstrates that polar solvent UAE can recover natural astaxanthin from seafood processing waste, offering an approach to valorizing shrimp shell byproduct.

Limits

The study is entirely in vitro and bench-scale with no biological, toxicological, or in vivo bioavailability testing. Only two time durations (2 and 4 h) were evaluated, and specific ultrasound parameters (power, frequency, temperature control) and economic feasibility were not reported in the abstract.

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