Holehan · Mechanisms of ageing and development 1985 · comparative longitudinal animal experiment · n=?

Lifetime breeding studies in fully fed and dietary restricted female CFY Sprague-Dawley rats. 1. Effect of age, housing conditions and diet on fecundity.

Cited 64 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal research with no human data.

PubMed 4079476 · doi:10.1016/0047-6374(85)90106-x · record verified 2026-08-30

What was done

Female CFY Sprague-Dawley rats were evaluated for their ability to bear multiple litters across their lifespan when breeding commenced at increasing ages. The study compared five conditions: continuous cohabitation with a male (GC), intermittent breeding in group-housed fully fed rats (GI), intermittent breeding in individually housed fully fed rats (I), dietary restriction (DR), and refeeding following dietary restriction (DRA). Measured outcomes included the percentage of females pregnant, number of litters produced, and total litter size.

What was found

Individual housing did not affect the percentage of females pregnant or the number of litters produced, but caused a significantly steeper decline in total litter size with maternal age compared to group housing (GI), matching the regression slope of continuous breeding (GC). Dietary restriction reduced litter size and overall fertility but extended breeding capacity up to 937 days of age, compared to a maximum of 720 days in all fully fed groups, with no age-related decline in litter size while restricted. Refeeding (DRA) restored the rate of litter size decline to that of GI rats; all DRA cohorts produced up to three litters, with breeding initiated at 750 days yielding litters as late as 952 days. Exact numerical counts and percentages were not reported in the abstract.

Why it matters

The findings demonstrate that dietary restriction substantially delays reproductive senescence in female rodents, preserving fertility into advanced age at the expense of immediate fecundity.

Limits

This is an animal study in Sprague-Dawley rats, limiting direct applicability to human reproductive biology. The abstract omits sample sizes, exact dietary restriction percentages, numerical litter counts, confidence intervals, and specific statistical test metrics.

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