Koep · The Journal of physiology 2025 · Within-subject crossover physiological study · n=13

Preferential lactate metabolism in the human brain during exogenous and endogenous hyperlactataemia.

Cited 9 times in the scientific literature.

Level 3 - non-randomized controlled study

Controlled within-subject crossover physiological study in healthy humans without explicit mention of randomization.

PubMed 41037311 · doi:10.1113/JP289216 · record verified 2026-08-29

What was done

Thirteen healthy adults (6 females) underwent two matched hyperlactataemia protocols (~4 and ~8 mmol/L arterial lactate): passive intravenous sodium lactate infusion (exogenous) and cycling exercise (endogenous). Researchers combined radial arterial and internal jugular venous blood sampling with cerebral blood flow (CBF) measurements to determine cerebral metabolic rates of oxygen (CMRO2), glucose (CMR_Glc), and lactate (CMR_iLac).

What was found

Cerebral blood flow was higher during exogenous infusion than during exercise (P < 0.001), despite infusion causing systemic alkalosis (P < 0.001). Total CMRO2 remained unchanged across both conditions and lactate levels (P = 0.610). CMR_Glc decreased significantly with increasing lactate (P = 0.009) with no difference between exercise and infusion conditions (P = 0.373). CMR_iLac increased in a dose-dependent manner (P < 0.001) similarly across conditions (P = 0.972). At 8 mmol/L arterial lactate, circulating lactate accounted for 24% of total cerebral oxidative metabolism.

Why it matters

This study demonstrates that the healthy human brain readily shifts from glucose to lactate oxidation based strictly on circulating substrate concentration, regardless of whether hyperlactataemia is generated by physical exertion or passive infusion.

Limits

The study had a small sample size (n = 13) restricted to healthy adults, precluding generalization to clinical populations with impaired cerebral metabolism or older adults. The abstract does not specify whether protocol order was randomized, and only acute, short-term metabolic responses were evaluated.

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