Crouch · Cell reports 2026 · Cross-sectional comparative metagenomic study · n=532

The gut microbiome of a Northern Plains tribe is in transition between global Indigenous and industrialized populations.

Cited 0 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional comparative observational study

PubMed 42384485 · doi:10.1016/j.celrep.2025.116334 · record verified 2026-08-29

What was done

Shotgun metagenomics was used to profile the gut microbiomes of individuals from a Northern Plains tribe reservation and compare them against 12 global populations categorized by traditional, agrarian, or industrialized lifestyles, analyzing 532 samples in total.

What was found

Across 532 samples, the Northern Plains tribe microbiota exhibited greater bacterial and archaeal diversity than industrialized populations, but reduced diversity compared to traditional and agrarian populations. Compared to the general United States population, tribal microbiomes had higher counts of virulence factor and microbial defense genes and fewer CAZyme-encoding genes. The abstract reports no exact numerical values or effect sizes.

Why it matters

This research provides rare metagenomic data from a North American Indian population experiencing post-colonial dietary transitions, demonstrating an intermediate microbiome state between traditional and industrialized groups.

Limits

The study is observational and cross-sectional. The abstract does not specify the sample size breakdown between the tribal group and the 12 comparison populations, nor does it provide quantitative effect sizes, confidence intervals, or clinical health correlates.

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