Sapp · Frontiers in nutrition 2026 · randomized double-blind placebo-controlled crossover trial · n=16

Absorption kinetics of vitamins and minerals from a novel nutritional product in physically active adults: a randomized, double-blind, placebo-controlled crossover trial.

Cited 0 times in the scientific literature.

Level 2 - randomized trial

Randomized double-blind placebo-controlled crossover clinical trial

PubMed 42518997 · doi:10.3389/fnut.2026.1793264 · record verified 2026-08-27

What was done

Sixteen healthy, physically active adults (8 males, 8 females) participated in a randomized, double-blind, placebo-controlled crossover trial. After a 10-hour overnight fast, subjects consumed a single 13 g serving of AG1 or a taste- and appearance-matched placebo in water, separated by a 1-week washout period. Blood was drawn at 0, 30, 60, 90, 120, 180, 240, 360, and 480 minutes post-ingestion to assess pharmacokinetic parameters (AUC 0-480 min, Cmax, Tmax) of folate, calcium, zinc, vitamin C, biotin, nicotinamide, pyridoxine, riboflavin, thiamine, and hesperidin.

What was found

AG1 significantly increased AUC 0-480 min (p < 0.05) for folate, calcium, zinc, vitamin C, biotin, nicotinamide, riboflavin, thiamine, and hesperidin compared with placebo. Pyridoxine did not show a statistically significant increase (p = 0.1205). Exact pharmacokinetic numbers (concentrations, Cmax, Tmax) were not reported in the abstract. No serious adverse events occurred.

Why it matters

Nutrient interactions in complex multi-ingredient supplements may alter bioavailability; this trial provides pharmacokinetic evidence that most tested micronutrients and phytochemicals in the formulation are effectively absorbed acutely.

Limits

The sample size is very small (n = 16) and limited to healthy, physically active adults. Only acute single-dose pharmacokinetics over 8 hours were measured, not long-term tissue status or functional health outcomes. Absolute numerical pharmacokinetic values were omitted from the abstract.

Cited by