Waldhauser · Lancet (London, England) 1984 · cross-sectional study · n=89

Fall in nocturnal serum melatonin during prepuberty and pubescence.

Cited 305 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional observational study measuring hormone levels across age and pubertal stages

PubMed 6141425 · doi:10.1016/s0140-6736(84)90412-4 · record verified 2026-08-28

What was done

Morning (7:30 AM to 10:00 AM) and nighttime (11:00 PM to 1:00 AM) serum melatonin concentrations, along with nocturnal luteinizing hormone (LH), were measured in 89 children, adolescents, and young adults classified by age and pubertal stage.

What was found

Morning serum melatonin levels (generally 0-20 pg/ml) did not change with sexual maturation or age. Nighttime melatonin levels decreased significantly: - Prepubertal children under 7 years: 195 +/- 24 pg/ml (mean +/- SEM) - Prepubertal children aged 7 or older: 119 +/- 23 pg/ml - Young adults (puberty stage V): 49 +/- 4 pg/ml - By age: 210 +/- 35 pg/ml (ages 1-5), 133 +/- 17 pg/ml (ages 5-11), and 46 +/- 4 pg/ml (young adults) - Nocturnal LH concentrations varied inversely with nocturnal melatonin (r = -0.35).

Why it matters

This study provides early evidence of a progressive decrease in nocturnal melatonin secretion from early childhood through puberty, linking pineal activity to the timing of human sexual maturation.

Limits

The study is cross-sectional rather than longitudinal. Blood sampling was restricted to two specific time windows rather than 24-hour profiling, and the total sample size (n = 89) is relatively small when split across multiple age and pubertal groups.

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