Donovan · The American journal of physiology 1983 · Comparative animal study · n=?

Endurance training affects lactate clearance, not lactate production.

Cited 384 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal physiological experiment

PubMed 6401405 · doi:10.1152/ajpendo.1983.244.1.E83 · record verified 2026-08-30

What was done

Primed-continuous infusions of [2-3H]- and [U-14C]lactate were used to measure lactate kinetics in endurance-trained rats (running 2 h/day at 29.4 m/min up a 15% gradient) and untrained controls. Measurements were taken at rest, during easy exercise (13.4 m/min, 1% gradient), and during hard exercise (26.8 m/min, 1% gradient). Blood lactate concentration, lactate turnover rates, and metabolic clearance rates were determined.

What was found

Blood lactate in trained rats was 1.0 +/- 0.09 mM at rest, 1.64 +/- 0.21 mM during easy exercise, and 2.66 +/- 0.38 mM during hard exercise, compared with control values of 1.93 +/- 0.21 mM (easy) and 4.62 +/- 0.57 mM (hard). Lactate turnover with [U-14C]lactate rose from 214.0 +/- 17.0 mumol.kg-1.min-1 at rest to 390.3 +/- 31.6 at easy exercise and 518.1 +/- 56.4 at hard exercise, with no significant differences between trained and control animals across any condition. [2-3H]lactate turnover followed identical patterns but yielded values 90% higher than [U-14C]lactate. Metabolic clearance rate did not differ at rest (180.6 +/- 27.7 ml.kg-1.min-1), but trained rats showed 37% and 107% greater clearance rates than controls during easy and hard exercise, respectively.

Why it matters

This study showed that endurance training attenuates circulating lactate accumulation during exercise by enhancing its metabolic clearance from the blood rather than reducing its rate of production.

Limits

The study was conducted in a rodent model, limiting direct translation to human exercise physiology. Sample size (n) was not reported in the abstract, resting baseline lactate for control animals was omitted, and the specific tissues responsible for the increased clearance were not quantified.

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