Isley · The Journal of clinical investigation 1983 · within-subject crossover feeding trial · n=5

Dietary components that regulate serum somatomedin-C concentrations in humans.

Cited 436 times in the scientific literature.

Level 3 - non-randomized controlled study

Small prospective repeated-measures interventional study in healthy humans without explicit randomization reported

PubMed 6681614 · doi:10.1172/jci110757 · record verified 2026-08-30

What was done

Five normal-weight adult volunteers underwent 5-day fasts on three separate occasions, followed by 5 days of refeeding with three distinct diets: a normal control diet, an isocaloric protein-deficient diet (containing 32% of control protein), and a diet deficient in both protein and total energy. Serum somatomedin-C (IGF-1) and daily nitrogen balance were measured.

What was found

Serum somatomedin-C fell from a baseline mean of 1.85 +/- 0.39 U/ml to 0.67 +/- 0.16 U/ml after 5 days of fasting (P < 0.005). Refeeding with a normal diet raised levels to 1.26 +/- 0.20 U/ml. Refeeding with an isocaloric protein-deficient diet resulted in a significantly smaller recovery to 0.90 +/- 0.24 U/ml (P < 0.05). Refeeding with both protein and energy restriction caused levels to decrease further to 0.31 +/- 0.06 U/ml. Across fasting and refeeding states, somatomedin-C fluctuations correlated strongly with daily nitrogen balance (r = 0.90).

Why it matters

This study demonstrates that circulating somatomedin-C is highly sensitive to acute nutritional status in humans, requiring adequate intake of both calories and protein for maintenance and recovery, making it a potential biomarker for nutritional response.

Limits

The study included only five participants, did not state whether the refeeding sequence was randomized, examined only short-term 5-day periods, and did not evaluate isolated energy restriction without protein restriction.

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