Stumpf · Science (New York, N.Y.) 1982 · preclinical animal autoradiography study · n=?

Brain target sites for 1,25-dihydroxyvitamin D3.

Cited 225 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal laboratory research with no human data

PubMed 6977846 · doi:10.1126/science.6977846 · record verified 2026-08-26

What was done

Autoradiographic localization was performed in rats using 3H-labeled 1,25-dihydroxyvitamin D3 to detect binding sites across the central nervous system. Specificity of neuronal binding in the forebrain, hindbrain, and spinal cord was tested by competitive displacement with unlabeled 1,25-dihydroxyvitamin D3 versus 25-hydroxyvitamin D3.

What was found

The abstract reports no numerical data. It observed nuclear retention and concentration of radioactivity in select neurons of the rat forebrain, hindbrain, and spinal cord. This nuclear retention was prevented by treatment with unlabeled 1,25-dihydroxyvitamin D3, but not by 25-hydroxyvitamin D3.

Why it matters

This study provides early histological evidence that 1,25-dihydroxyvitamin D3 targets specific central nervous system neurons, suggesting direct central endocrine or neuromodulatory roles for vitamin D.

Limits

This is an animal study in rats, which limits direct generalizability to human neurobiology. The abstract reports no sample sizes, specific quantitative binding metrics, or functional physiological and behavioral outcomes.

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