Hotamisligil · Science (New York, N.Y.) 1993 · controlled animal experiment · n=?

Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance.

Cited 7486 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Bench and animal research with no human data

PubMed 7678183 · doi:10.1126/science.7678183 · record verified 2026-08-29

What was done

Researchers measured tumor necrosis factor-alpha (TNF-alpha) mRNA and protein expression in adipose tissue and systemically across four rodent models of obesity and diabetes. They also evaluated peripheral glucose uptake in response to insulin following neutralization of TNF-alpha in obese fa/fa rats.

What was found

TNF-alpha mRNA and protein were elevated locally in adipose tissue and systemically in four rodent models of obesity and diabetes. Neutralization of TNF-alpha in obese fa/fa rats resulted in a significant increase in peripheral glucose uptake in response to insulin. The abstract reports no exact numerical values or statistics.

Why it matters

This study provides mechanistic preclinical evidence directly linking adipose-derived TNF-alpha expression to obesity-induced insulin resistance.

Limits

The findings are derived entirely from rodent models with no human clinical data. The abstract lacks specific sample sizes, numerical effect sizes, variance estimates, and details on intervention duration.

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