MacDougall · Canadian journal of applied physiology = Revue canadienne de physiologie appliquee 1995 · within-subject controlled trial · n=6

The time course for elevated muscle protein synthesis following heavy resistance exercise.

Cited 247 times in the scientific literature.

Level 2 - randomized trial

Randomized within-subject controlled physiological trial

PubMed 8563679 · doi:10.1139/h95-038 · record verified 2026-08-26

What was done

Six healthy young men performed 12 sets of 6- to 12-RM elbow flexion exercises with one arm, while the contralateral arm served as an unexercised control. Muscle protein synthesis (MPS) was measured in the biceps brachii of both arms at an average of 36 hours post-exercise using primed constant infusion of L-[1,2-13C2] leucine over 11 hours.

What was found

At an average of 36 hours post-exercise, MPS in the exercised arm had returned to within 14% of the control arm value, with the difference being statistically non-significant.

Why it matters

This study delineates the duration of the post-exercise anabolic response, indicating that the marked elevation in muscle protein synthesis largely subsides by 36 hours.

Limits

The sample size was very small (n = 6) and restricted solely to healthy young men. The exercise was limited to isolated elbow flexion, and the abstract reports no data on nutritional status or earlier intermediate timepoints in this specific cohort.

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