Irwin · FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1996 · prospective pre-post experimental study · n=42

Partial night sleep deprivation reduces natural killer and cellular immune responses in humans.

Cited 486 times in the scientific literature.

Level 3 - non-randomized controlled study

Prospective non-randomized within-subject experimental trial

PubMed 8621064 · doi:10.1096/fasebj.10.5.8621064 · record verified 2026-08-31

What was done

Forty-two medically and psychiatrically healthy male volunteers underwent early-night partial sleep deprivation (kept awake from 10:00 P.M. to 3:00 A.M.) followed by a night of recovery sleep. Researchers evaluated circulating white blood cell counts, natural killer (NK) cell count and cytotoxicity, lymphokine-activated killer (LAK) cell count and activity, and concanavalin A-stimulated interleukin-2 (IL-2) production at baseline, after sleep deprivation, and after recovery sleep.

What was found

The abstract reports directional findings without providing numerical values, variance, or p-values. Partial sleep deprivation reduced NK cell activity, NK activity per NK cell, LAK activity, LAK activity per precursor cell (CD16,56, CD25), and concanavalin A-stimulated IL-2 production (attributable to changes in both adherent and nonadherent cells). Following a night of recovery sleep, NK activity returned to baseline levels, whereas IL-2 production remained suppressed.

Why it matters

These findings show that even modest, acute sleep loss can transiently suppress human innate cellular cytotoxicity and T-cell cytokine production.

Limits

The abstract reports no numerical values, effect sizes, or statistical confidence intervals. The sample consisted solely of healthy adult men, precluding generalization to women, older adults, or clinical populations. Additionally, there was no parallel non-deprived control group to rule out potential circadian or protocol-confinement confounders.

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