Fattinger · The Journal of pharmacology and experimental therapeutics 1997 · uncontrolled pharmacodynamic infusion study · n=9

Pharmacodynamics of acute tolerance to multiple nicotinic effects in humans.

Cited 55 times in the scientific literature.

Level 4 - case-series / case-control

Uncontrolled experimental pharmacodynamic study in a single small human cohort

PubMed 9190859 · record verified 2026-08-29

What was done

Nine healthy smokers abstaining from tobacco received a rapid intravenous nicotine infusion to reach a target plasma concentration of approximately 25 ng/ml, maintained via computer-controlled infusion. Researchers tracked multiple physiological and biochemical responses—including heart rate, blood pressure, plasma epinephrine, energy expenditure, and free fatty acid levels—and applied a semiparametric pharmacodynamic model to evaluate the development of acute tolerance.

What was found

Acute tolerance developed for heart rate, blood pressure, plasma epinephrine, and energy expenditure within nicotine levels typical of smokers. The rate of tolerance development varied widely across endpoints: tolerance half-lives ranged from 3.5 minutes for energy expenditure to 70 minutes for systolic blood pressure. No apparent tolerance occurred for free fatty acid concentrations (lipolysis).

Why it matters

This study demonstrates that human tolerance to nicotine is effect-specific rather than uniform, pointing to different desensitization kinetics or distinct mechanisms across nicotinic receptor subtypes.

Limits

The sample size was very small (n = 9) and restricted to healthy smokers, without a placebo control group. The abstract does not provide numerical values or variance for heart rate or epinephrine tolerance rates.

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