Fahey · Proceedings of the National Academy of Sciences of the United States of America 1997 · Preclinical animal study and biochemical assay · n=?

Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens.

Cited 1385 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal model and laboratory biochemical assays without human participants.

PubMed 9294217 · doi:10.1073/pnas.94.19.10367 · record verified 2026-08-26

What was done

Researchers quantified phase 2 detoxication enzyme-inducing glucosinolates (glucoraphanin) in 3-day-old cruciferous sprouts (broccoli and cauliflower) versus mature plants using a low-temperature multi-solvent extraction protocol. They also tested the ability of 3-day-old broccoli sprout extracts to inhibit dimethylbenz(a)anthracene (DMBA)-induced mammary tumorigenesis in a rat model.

What was found

Three-day-old sprouts of certain broccoli and cauliflower cultivars contained 10- to 100-fold higher levels of glucoraphanin than mature plants. Extracts of these sprouts reduced the incidence, multiplicity, and rate of development of mammary tumors in DMBA-treated rats (numerical values were not reported in the abstract). Sprouts also contained negligible amounts of indole glucosinolates compared to mature vegetables.

Why it matters

This paper demonstrated that 3-day-old broccoli sprouts are an exceptionally rich source of phase 2 enzyme inducers (specifically sulforaphane precursors), suggesting that small dietary quantities of young sprouts could offer anticarcinogenic effects comparable to much larger amounts of mature vegetables.

Limits

The study is entirely preclinical (in vitro biochemical assays and a rat carcinogenesis model) with no human clinical data. The abstract does not provide sample sizes, quantitative tumor rates, effect sizes, variance estimates, or safety data.

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