Noemí Ruiz‐López · Journal of Experimental Botany 2012 · narrative review · n=?

Metabolic engineering of the omega-3 long chain polyunsaturated fatty acid biosynthetic pathway into transgenic plants

Cited 147 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Oxford CEBM level 5; narrative review of plant metabolic engineering and biochemistry without human clinical data.

OpenAlex W2142038669 · doi:10.1093/jxb/err454 · record verified 2026-08-30

What was done

This narrative review summarizes research on metabolically engineering the omega-3 very long-chain polyunsaturated fatty acid (VLC-PUFA) biosynthetic pathway into transgenic oilseed crops to produce eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It examines gene identification, pathway reconstitution, lipid remodelling, and technical tools such as lipidomics and next-generation sequencing.

What was found

The abstract reports no numerical data or specific production yields. It notes qualitatively that reconstitution of the VLC-PUFA biosynthetic pathway has enabled transgenic plants to accumulate omega-3 VLC-PUFAs at levels approaching those in native marine organisms, while advancing knowledge of acyl exchange and lipid remodelling mechanisms.

Why it matters

Providing a plant-based terrestrial source of EPA and DHA could alleviate commercial pressure on depleted marine fish stocks and mitigate concerns regarding marine pollutants in dietary fish oil.

Limits

The abstract describes a narrative review without quantitative meta-analytic data, comparative crop yield metrics, stability assessments across field generations, or evaluation of human bioequivalence and safety.

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