DNA sequencing: bench to bedside and beyond
Level 5 - mechanism / opinion, no new human data
Narrative historical review with no primary empirical data (design analogy).
OpenAlex W2160265043 · doi:10.1093/nar/gkm688
What was done
This paper is a historical narrative review tracing the evolution of DNA sequencing technologies. It covers the timeline from the discovery of the double helix and early sequencing in 1968, the introduction of Maxam-Gilbert and Sanger dideoxy methods in 1977, the scale-up during the Human Genome Project, to the emergence of massively parallel sequencing platforms.
What was found
The abstract reports a historical overview without primary quantitative experimental data. Key historical milestones noted include: a 15-year gap between 1953 and initial sequencing in 1968; modern sequencing initiation in 1977 with phage phiX174; scaling to targets >200 kb (human cytomegalovirus); establishment of high-throughput sequencing facilities by 1992; first cellular bacterial genomes in 1995; draft human genome sequences published in 2001; and the ongoing pursuit of the 'thousand dollar genome' via massively parallel sequencing.
Why it matters
It contextualizes the technological and computational transitions that enabled modern genomics, bioinformatics, and early efforts toward personalized medicine.
Limits
The paper is a narrative overview containing no original experimental data, systematic methodology, or quantitative benchmarking of sequencing platforms.
Cited by
- supports A draft of the human genome was completed around 2000 through an international multi-billion-dollar effort.