Genome‐scale engineering for systems and synthetic biology
Level 5 - mechanism / opinion, no new human data
Level 5 by design analogy (narrative review/expert perspective on biotechnology methods).
OpenAlex W2167299815 · doi:10.1038/msb.2012.66
What was done
This is a narrative review evaluating technologies and methodologies for genome-scale engineering in systems and synthetic biology. The authors assess methods for directed and combinatorial cellular engineering, discuss approaches for expanding genome modification tools into new host organisms, and review applications in programmable chasses, synthetic biochemistries, and ecological containment.
What was found
The abstract reports no quantitative data or numerical outcomes. It describes a qualitative transition from historical, labor-intensive techniques restricted to random or isolated targeted edits toward modern platforms capable of efficient, multiplexed, and genome-wide engineering.
Why it matters
It provides a conceptual roadmap for how high-throughput genome editing enables rational reprogramming of biological systems and whole organisms.
Limits
The work is a non-systematic narrative review providing theoretical and technological overview rather than primary experimental data or statistical pooling. The abstract lacks quantitative comparisons of editing efficiency, fidelity, or organism-specific performance.
Cited by
- supports The first completely recoded organism genome was engineered primarily using single-stranded DNA-annealing proteins (SSAPs) and site-specific recombinases.