Paddon-Jones · The Journal of clinical endocrinology and metabolism 2004 · Randomized controlled trial · n=?

Essential amino acid and carbohydrate supplementation ameliorates muscle protein loss in humans during 28 days bedrest.

Cited 340 times in the scientific literature.

Level 2 - randomized trial

Individual randomized controlled trial

PubMed 15356032 · doi:10.1210/jc.2003-032159 · record verified 2026-08-27

What was done

Participants underwent 28 days of bed rest and were assigned to a control group receiving standard nutritionally mixed meals every 5 hours or an experimental group receiving the standard diet supplemented with 16.5 g of essential amino acids and 30 g of carbohydrate three times daily (at 1100, 1600, and 2100 h). On days 1 and 28, mixed muscle fractional synthetic rate (FSR), phenylalanine net balance, lean leg mass, and leg extension strength were evaluated during a 16-hour tracer infusion using vastus lateralis muscle biopsies and femoral arterio-venous blood sampling.

What was found

Muscle fractional synthetic rate was higher in the supplement group on day 1 (0.099 ± 0.008%/h vs. 0.075 ± 0.005%/h in controls) and day 28 (0.093 ± 0.006%/h vs. 0.055 ± 0.007%/h). Lean leg mass was maintained in the supplement group (+0.2 ± 0.3 kg) but decreased in controls (-0.4 ± 0.1 kg). Leg extension strength decreased in both groups, but the loss was smaller with supplementation (-8.8 ± 1.4 kg vs. -17.8 ± 4.4 kg in controls).

Why it matters

This study demonstrates that targeted essential amino acid and carbohydrate supplementation can preserve lean mass and attenuate muscle wasting and weakness caused by prolonged disuse.

Limits

The abstract does not state the total sample size, participant demographics (such as age or sex), or p-values. Phenylalanine net balance values are mentioned as an outcome but specific numerical results are omitted.

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