Johnston · Nutrition & metabolism 2006 · Cross-sectional comparison and randomized double-blind trial · n=22

Marginal vitamin C status is associated with reduced fat oxidation during submaximal exercise in young adults.

Cited 61 times in the scientific literature.

Level 2 - randomized trial

Double-blind, placebo-controlled interventional trial following a cross-sectional assessment

PubMed 16945143 · doi:10.1186/1743-7075-3-35 · record verified 2026-08-30

What was done

Fat energy expenditure was measured during a 60-minute submaximal treadmill test in 22 young adults with either marginal (n = 15) or adequate (n = 7) vitamin C status. Subsequently, 8 participants with marginal vitamin C status completed an 8-week double-blind, placebo-controlled depletion-repletion trial evaluating daily supplementation with 500 mg vitamin C versus placebo during submaximal exercise testing.

What was found

Participants with marginal vitamin C status oxidized 25% less fat per kg body weight during the treadmill test compared to those with adequate status. Fat oxidation during exercise was inversely correlated with fatigue (r = -0.611, p = 0.009). In the intervention phase, 500 mg/day vitamin C repletion increased fat energy expenditure during exercise 4-fold compared to depleted controls (p = 0.011).

Why it matters

These findings suggest that suboptimal vitamin C intake directly impairs fatty acid oxidation during exercise, identifying a potential metabolic mechanism linking low vitamin C status with elevated adiposity and difficulty losing weight.

Limits

The total sample size was very small (n = 22 for the cross-sectional phase, n = 8 for the interventional phase). The abstract does not report baseline fitness levels, sex distribution, dietary carnitine intake, or actual body weight changes over time.

Cited by