Ceriello · Diabetes research and clinical practice 2008 · Controlled physiological clamp study · n=?

Glucose "peak" and glucose "spike": Impact on endothelial function and oxidative stress.

Cited 122 times in the scientific literature.

Level 3 - non-randomized controlled study

Non-randomized controlled physiological human interventional study (clamp experiment)

PubMed 18950890 · doi:10.1016/j.diabres.2008.07.015 · record verified 2026-08-27

What was done

Investigators conducted stepwise euinsulinemic hyperglycemic clamps targeting 10 and 15 mmol/l glucose levels, with and without concurrent vitamin C infusion, in individuals with diabetes and healthy control subjects. In a second experiment, a 10 mmol/l hyperglycemic clamp was administered to two groups of diabetic patients categorized by different baseline fasting glucose concentrations. Endothelial function was assessed via flow-mediated dilation, and oxidative stress was evaluated by measuring plasma nitrotyrosine levels.

What was found

The abstract reports no numerical values, baseline participant characteristics, sample sizes, or p-values. Glucose elevations to 10 and 15 mmol/l produced concentration-dependent reductions in flow-mediated dilation and increases in nitrotyrosine, an effect counterbalanced by co-administration of vitamin C. Raising glucose to 10 mmol/l impaired endothelial function and elevated nitrotyrosine across both diabetic cohorts. When assessed as the change (delta) from baseline, patients starting with lower baseline fasting glucose experienced greater relative worsening in endothelial function and oxidative stress markers.

Why it matters

These findings suggest that the magnitude of an acute glucose surge relative to fasting levels (the glucose spike) may drive vascular oxidative stress and endothelial impairment, rather than absolute post-challenge glucose concentrations alone.

Limits

The abstract omits sample size, participant demographics, standard deviation metrics, and exact quantitative results. The study design lacks details regarding randomization or blinding, and the acute clamp setting does not establish long-term clinical cardiovascular outcomes.

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