Massip · FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 · Controlled animal experiment · n=?

Vitamin C restores healthy aging in a mouse model for Werner syndrome.

Cited 115 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal research with no human participants.

PubMed 19741171 · doi:10.1096/fj.09-137133 · record verified 2026-08-30

What was done

Researchers evaluated Wrn helicase-domain mutant mice (a model for Werner syndrome) and wild-type controls to assess premature aging phenotypes, including liver sinusoidal endothelial defenestration, inflammation, and metabolic syndrome. They tested the effects of vitamin C supplementation on mouse lifespan, adipose and liver pathology, genomic integrity, inflammatory markers, molecular stress pathways (including Akt substrates, NF-kappaB, PKCdelta, Hif-1alpha, and PPARalpha), and liver gene expression profiles via microarray and gene set enrichment analyses.

What was found

The abstract reports no numerical values, life span extensions, effect sizes, or p-values. It qualitatively states that vitamin C supplementation rescued the shortened mean life span of Wrn mutant mice and reversed age-related abnormalities in adipose tissues, liver endothelial defenestration, genomic integrity, and inflammatory status. In Wrn mutant liver tissue, vitamin C normalized elevated levels of phosphorylated Akt substrates, NF-kappaB, PKCdelta, and Hif-1alpha, and increased PPARalpha. It also decreased expression of genes typically up-regulated in human Werner syndrome fibroblasts and cancers. Vitamin C had no such effects in wild-type mice.

Why it matters

The study suggests that vitamin C can counteract metabolic and tissue-specific dysfunction in a genetic model of Werner syndrome, offering a mechanistic basis for testing antioxidant strategies in premature aging.

Limits

The study was conducted entirely in mice, which may not accurately reflect human Werner syndrome physiology or therapeutic response. The abstract lacks specific sample sizes (n), treatment dosages, duration of supplementation, and quantitative statistical metrics. Healthy wild-type animals experienced no benefit, indicating specificity only to this severe mutant phenotype.

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